Recently Updated NPI Information — some details in this NPI profile have been updated in the NPI registry within the last 60 days.

TWISTED ROOTS HEALTHCARE
NPI 1942169966
Nurse Practitioner - Family in Johnson City, TN

Active since January 16, 2026CLIA 44D2337544 · Microscopy
2020 NORTHPARK DR STE 2C, JOHNSON CITY, TN 37604(423) 367-7688(877) 682-3703 Get Directions Write a Review

NPPES record last updated: September 17, 2026. Verified against the NPPES registry weekly; last sync: October 18, 2026.

Record update history: Sep 17, 2026, Apr 15, 2026, Jan 27, 2026 and 1 more (4 updates tracked since 2026).

About Twisted Roots Healthcare NPPES

Official registry information on file with the National Plan and Provider Enumeration System.

TWISTED ROOTS HEALTHCARE (NPI 1942169966) is a healthcare organization registered as a family in Johnson City, Tennessee and active in the NPI registry since January 2026. The organization holds a CLIA Microscopy certificate valid through January 25, 2028 and lists Eva M Jessee, Owner, as its authorized official.

NPPES Registry Identity

NPI1942169966
Entity TypeOrganization
Primary Taxonomy363LF0000X
Legal Business NameTWISTED ROOTS HEALTHCARE
Location Address2020 NORTHPARK DR STE 2CJohnson City, TN 37604-3127
Mailing Address823 Liberty Church RdKingsport, TN 37663-4614 · (423) 276-1298 · Fax (877) 682-3707
Fax(877) 682-3703
Organization SubpartNo
Authorized OfficialEva M JesseeOwner · (423) 276-1298
Enumeration DateJanuary 16, 2026
Last NPPES UpdateSeptember 17, 20264 updates tracked since enumeration
NPPES CertifiedSeptember 17, 2026
✔ NPI 1942169966 is a valid, active identifier and passes the ISO check-digit test.

Specialties & Licenses 3

★ Primary SpecialtyNurse Practitioner · FamilyPhysician Assistants & Advanced Practice Nursing Providers
Taxonomy Code363LF0000X
Also ListedClinic/Center · Urgent CareTaxonomy 261QU0200X
Also ListedNurse Practitioner · Community HealthTaxonomy 363LC1500X
2020 NORTHPARK DR STE 2C, Johnson City, TN 37604

Other Names 1

Other NameTwisted Roots Healthcare, Pllc

Medicare Participation & PECOS CMS

Medicare enrollment and the clinicians who bill Medicare through this organization, from CMS.

Medicare Enrollment Status

✔

Enrolled in Medicare (PECOS)

Twisted Roots Healthcare is registered in the CMS Provider Enrollment, Chain and Ownership System (PECOS).

Clinicians Billing Medicare Through This Organization

Medicare enrollment records show 1 clinician reassigns Medicare billing to Twisted Roots Healthcare, which bills Medicare for the services this clinician delivers.

Clinician (1)

Nurse Practitioner
NPI 1750248852
2020 NORTHPARK DR STE 2C
Johnson City, TN 37604
(423) 367-7688
✔ PECOSSame address

Source: CMS Revalidation Reassignment List, October 2026 release. Reassignment records can lag staffing changes; confirm a current affiliation with the organization.

CLIA Laboratory Certificates CMS CLIA

Laboratory certificates issued under the Clinical Laboratory Improvement Amendments (CLIA) program, which sets federal quality standards for human laboratory testing in the United States. There is no official CMS crosswalk between CLIA and NPI numbers; these certificates are associated with this NPI by matching facility names, locations and phone numbers in the CMS CLIA registry.

Certificate for Provider-Performed Microscopy Procedures (PPMP) 44D2337544

Twisted Roots Healthcare · Johnson City, TN
Active · expires Jan 25, 2028
CLIA Number44D2337544
Laboratory TypePhysician Office
Certificate Effective DateJanuary 26, 2026
Certificate Expiration DateJanuary 25, 2028
Laboratory DirectorEva M. Jessee
Laboratory Phone(423) 367-7688
Laboratory LocationTwisted Roots Healthcare2020 Northpark Drive, Ste 2C, Johnson City, TN 37604
CLIA data matches this NPI record on: Address Name Phone
This certificate is issued to a laboratory in which a physician, midlevel practitioner or dentist performs no tests other than the microscopy procedures. This certificate permits the laboratory to also perform waived tests.

Frequently Asked Questions NPPES & CMS

Common questions about this NPI record, answered from the official NPPES registry and CMS datasets shown on this page.

What is Twisted Roots Healthcare's NPI number?

The NPI number for Twisted Roots Healthcare is 1942169966. It was assigned to this organization in the NPPES registry on January 16, 2026. The provider is also known as Twisted Roots Healthcare, PLLC.

Where is Twisted Roots Healthcare located?

Twisted Roots Healthcare is located at 2020 Northpark Dr Ste 2C, Johnson City, TN 37604. The listed phone number is (423) 367-7688.

What is Twisted Roots Healthcare's specialty?

The primary specialty registered for this NPI is Nurse Practitioner, specializing in Family, with taxonomy code 363LF0000X.

Is Twisted Roots Healthcare enrolled in Medicare?

Yes. Twisted Roots Healthcare is registered in the Medicare PECOS enrollment system.

How many clinicians bill Medicare through Twisted Roots Healthcare?

According to the CMS Revalidation Reassignment List (October 2026 release), 1 clinician (Nurse Practitioner) reassigns their Medicare billing to Twisted Roots Healthcare. A reassignment is filed with Medicare on form CMS-855R, signed by both the clinician and the organization, and lets the organization bill Medicare and receive payment for the clinician's services. Reassignment records can lag staffing changes; confirm a current affiliation with the organization.

Does Twisted Roots Healthcare hold a CLIA laboratory certificate?

Yes. A Certificate for Provider-Performed Microscopy Procedures (PPMP) (number 44D2337544) is associated with this NPI, valid through January 25, 2028. CLIA certificates authorize laboratory testing under the federal Clinical Laboratory Improvement Amendments program.

When was this NPI record last updated?

The NPPES record for Twisted Roots Healthcare was last updated on September 17, 2026. NPI Profile syncs weekly with the NPPES registry data releases published by CMS. This NPI record was last updated 34 days ago. If this record has changed at CMS more recently, you can request an on-demand re-check against the live CMS registry, directly from this page.